Workflow 3

Workflow 3: Interspecific coexistence and niche partitioning in phytoplankton

Workflow 3 is dedicated to the study of interspecific coexistence mechanisms in phytoplankton communities, using a size–abundance relationship approach and analysing how changes in abiotic factors influence niche partitioning. The workflow supports the use of laboratory, field, and monitoring data, including species frequency and absolute abundance, body size, and abiotic environmental variables.

This workflow provides a reproducible framework to investigate species coexistence and niche partitioning in phytoplankton communities. It includes the selection of dominant taxa, the integration of biological and environmental data, and the grouping of taxa into body-size classes. Multivariate ordination analyses, such as Canonical Correspondence Analysis (CCA), are used to describe species distributions along environmental gradients and to derive niche position and niche breadth metrics. The workflow further quantifies niche overlap within and among size classes and applies null model Monte Carlo simulations to assess whether observed patterns of niche partitioning differ from random expectations.

Thanks to its modular structure, this workflow is flexible and easily adaptable. Users can customize the workflow by editing the code and parameters and by adding or removing cells. Furthermore, the workflow can be tailored to specific research needs by performing additional analyses.